Optimized protocol for conditioned place avoidance learning in juvenile zebrafish
نویسندگان
چکیده
منابع مشابه
Conditioned Place Avoidance of Zebrafish (Danio rerio) to Three Chemicals Used for Euthanasia and Anaesthesia
Zebrafish are becoming one of the most used vertebrates in developmental and biomedical research. Fish are commonly killed at the end of an experiment with an overdose of tricaine methanesulfonate (TMS, also known as MS-222), but to date little research has assessed if exposure to this or other agents qualifies as euthanasia (i.e. a "good death"). Alternative agents include metomidate hydrochlo...
متن کاملInhibitory Avoidance Learning in Zebrafish (Danio rerio)
The zebrafish (Danio rerio) is increasingly being used as model in behavioural [1,2], neurobiological and genetic research [3]. Underlying reasons are high genetic homology to humans [4] and the many advantages over the use of rodents, such as low cost, easy handling, short reproduction cycle and high fecundity. Furthermore, its genome [5], transcriptome [6] and proteome [7] are well described,...
متن کاملConditioned Avoidance
The procedure described here involves training an animal to make steady contact with a reward spout in order to receive food or water and then pairing a stimulus with mild electric shock delivered through the spout. The animal quickly learns to avoid the shock by breaking contact with the spout whenever it detects the stimulus. The breaking of contact with the spout is then used to indicate tha...
متن کاملAssessing the Value of the Zebrafish Conditioned Place Preference Model for Predicting Human Abuse Potential.
Regulatory agencies recommend that centrally active drugs are tested for abuse potential before approval. Standard preclinical assessments are conducted in rats or non-human primates (NHPs). This study evaluated the ability of the zebrafish conditioned place preference (CPP) model to predict human abuse outcomes. Twenty-seven compounds from a variety of pharmacological classes were tested in ze...
متن کاملBehavioral and Molecular Analysis of Nicotine-Conditioned Place Preference in Zebrafish
Studies using mice and rats have demonstrated that nicotine induces a conditioned place preference (CPP), with more effective results obtained by using biased procedures. Zebrafish have also been used as a model system to identify factors influencing nicotine-associated reward by using an unbiased design. Here, we report that zebrafish exhibited putative nicotine biased CPP to an initially aver...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: STAR Protocols
سال: 2021
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2021.100465